Paternity determination in captive lowland gorillas and orangutans and wild mountain gorillas by microsatellite analysis |
| |
Authors: | Dawn Field Leona Chemnick Martha Robbins Karen Garner Oliver Ryder |
| |
Institution: | (1) Center for Reproduction of Endangered Species, Zoological Society of San Diego, P. O. Box 551, 92112-0551 San Diego, California, USA;(2) University of California, San Diego, 92037 La Jolla, California, USA;(3) Department of Zoology, Birge Hall 430 Lincoln Drive, University of Wisconsin, 53706 Madison, Wisconsin, USA;(4) USDA Forest Service, 359 Main Road, 43015 Delaware, Ohio, USA |
| |
Abstract: | Paternity exclusion studies provide useful information for testing certain theories of behavioral ecology and for the management
and conservation of both wild and captive populations of endangered species. This study used eight human nuclear microsatellite
loci, in the absence of species-specific PCR primers, to genetically identify the sires of 12 captive lowland gorillas (Gorilla gorilla gorilla) and 2 captive orangutans (Pongo pygmaeus pygmaeus andPongo p. abelii). Parentage assignments were confirmed by excluding all except a single potential sire for each offspring with the least
two loci. Sire-offspring relationships were verified in 12 of the 14 cases, and reassigned in the case of two gorilla offspring.
The orangutan paternity typing was supplemented by DNA fingerprinting. Additionally, five of the eight microsatellite loci,
in conjunction with behavioral data, were used for a non-exhaustive set of paternity exclusions for five wild mountain gorillas
(Gorilla g. beringei). The eight loci described in this study should be useful additions to the tools available for the study of genetics in the
great apes. |
| |
Keywords: | Paternity testing Microsatellites Gorilla gorilla Pongo pygmaeus DNA typing |
本文献已被 SpringerLink 等数据库收录! |
|