Axial ligand replacement in horse heart cytochrome c by semisynthesis |
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Authors: | A L Raphael H B Gray |
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Affiliation: | Arthur Amos Noyes Laboratory, California Institute of Technology, Pasadena 91125. |
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Abstract: | Semisynthesis has been employed to replace the axial methionine in horse heart cytochrome c with histidine. The reduction potential of the His-80 protein (cyt c-His-80) is 41 mV vs NHE (0.1 M phosphate; pH 7.0; 25 degrees C). The absorption spectra of oxidized and reduced cyt c-His-80 are very similar to those of the native protein in the porphyrin region, but the 695 nm band is absent in the oxidized His-80 protein. |
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Keywords: | cytochrome c axial ligand semisynthesis |
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