Use of evolutionary operation (EVOP) factorial design technique to develop a bioprocess using grease waste as a substrate for lipase production |
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Authors: | Kumar Sunil Katiyar Nitin Ingle Priyanka Negi Sangeeta |
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Institution: | Biotechnology Section, Applied Mechanics Department, Motilal Nehru National Institute of Technology, Allahabad 211 004, Uttar Pradesh, India |
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Abstract: | The aim of the present work was to develop a bioprocess using EVOP-factorial design technique employing grease waste as a substrate for the production of lipase. A newly isolated fungal strain of Penicillium chrysogenum was explored for the fermentation process. Solid-state fermentation (SSF) was carried out using grease waste and Czapek-dox medium, supplemented with wheat bran. The yield of lipase was 38 U/ml when SSF was carried out at 32 °C for 8 days and grease:wheat bran:Czapek-dox media in 1:1:2 (w/w/v). Different physicochemical parameters affecting the production of lipase were optimized through evolutionary operation (EVOP) factorial design technique and after optimization yield was enhanced up to 46 U/ml at 30 °C, pH 7.0 with 1:1:2 (w/w/v) grease waste:wheat bran:Czapek-dox media. Industrial grease waste has never been reported before for the production of industrially important lipase enzyme. |
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Keywords: | Solid-state fermentation EVOP Lipase Grease waste Penicillium chrysogenum |
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