Ethanol-resistant mutants of Nicotiana plumbaginifolia are deficient in the expression of pollen and seed alcohol dehydrogenase activity |
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Authors: | Philippe Rousselin Andrée Lepingle Jean-Denis Faure Rémy Bitoun and Michel Caboche |
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Institution: | (1) Laboratoire de Biologic Cellulaire, INRA Centre de Versailles, F-78026 Versailles Cedex, France |
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Abstract: | Summary Six independent mutant lines ofNicotiana plumbaginifolia resistant to ethanol, designated E3, E8, E101, E112, E144 and E251, were isolated as germinating seedlings on selective medium. In all cases, resistance to ethanol was conferred by a single recessive nuclear mutation at the same locus. Mutant seeds and pollen lacked detectable ADH activity, with the exception of E251 where a residual activity was detected. An antiserum directed againstArabidopsis thaliana ADH detected an ADH-related polypeptide of 44 kDa present in wild-type seeds and, to a lesser extent, in the seeds of the leaky mutant E251. No ADH-related polypeptide could be detected in seeds of the other mutants. However, all of them had a nearly normal level of ADH mRNA except one which did not synthesize any mRNA. These results suggest that these ethanol-resistant mutants are impaired in one of the structural genes coding for alcohol dehydrogenase. The corresponding locus has been designatedAdh1.Abbreviations ADH
alcohol dehydrogenase
- EMS
ethyl methane-sulfonate
- MTT
dimethyl thiazol tetrazolium
- NAD
nicotinamide adenine dinucleotide
- NBT
nitro blue tetrazolium
- p-cells
protoplast-derived cells
- PMS
phenazine methosulfate
- SDS
sodium dodecyl sulfate |
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Keywords: | Mutagenized seeds Nicotiana plumbaginifolia Ethanol selection Alcohol dehydrogenase |
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