Some kinetic properties of a purified glutamine synthetase from bacteroids of Glycine max |
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Authors: | Basant Bhandari D.J.D. Nicholas |
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Affiliation: | Agricultural Biochemistry Department, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, 5064, South Australia, Australia |
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Abstract: | Abstract A purified glutamine synthetase was prepared from bacteroids of Rhizobium japonicum from nodules of Glycine max . For the biosynthetic assay the K m values (mM) were l -glutamate 12.9, NH4Cl 8.9 and ATP 14.3. When the enzyme was assayed by the γ-glutamyltransferase reaction the K m values (mM) were l -glutamine 11.1 and hydroxylamine 3.3 compared with 7.7 and 1.2, respectively, for the purified enzyme from Rhizobium japonicum grown in culture. The enzyme prepared from bacteroids of Glycine max was 80% adenylylated. |
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Keywords: | Glutamine synthetase kinetic properties bacteroids Rhizobium japonicum Glycine max |
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