Cloning and molecular genetic analysis of the frd operon encoding the fumarate reductase of Proteus vulgaris |
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Authors: | Nadine Honoré Stewart T Cole |
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Institution: | Groupement de Génie Génétique, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France |
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Abstract: | Abstract The frd operon encoding the fumarate reductase of Proteus vulgaris has been cloned and a restriction map of the flanking chromosomal region established. Although the cloned genes complement an Escherichia coli frdA mutant, the overall arrangement of the P. vulgaris chromosome near the frd operon is very different. None of the well-characterised markers linked to the E. coli frd genes, such as ampC or mop , could be detected by genetic studies or DNA-DNA hybridisation. |
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Keywords: | Iron-sulphur flavoenzyme anaerobic electron transfer cephalosporinase gene evolution |
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