Acetyl esterase from mediterranean oranges: partial purification, immobilisation and biotransformations |
| |
Authors: | Piero Pasta Roberto Verga Francesca Zambianchi Moreno Daminati |
| |
Institution: |
a Istituto di Chimica del Riconoscimento Molecolare CNR, Milano, Italy
b Antibioticos S.p.A., Rodano (Milano), Italy
c Resindion S.r.l., Binasco (Milano), Italy |
| |
Abstract: | Acetyl esterase (acetic-ester acetylhydrolase, EC 3.1.1.6) from citrus peel, whose natural role is not well known, catalyses, in vitro, the hydrolysis of acetyl groups from a wide range of substrates. This enzyme was extracted from Mediterranean orange peel, largely available in Italy, and purified 190-fold by a single chromatographic step on Sepabeads FP-HG. SDS polyacrylamide gel electrophoresis of the purified enzyme showed a major protein band, corresponding to a molecular mass of 45 kDa. Both free and immobilised enzyme were used in biotransformations. The enzyme removed the acetyl group in the 3 position of β-lactamic antibiotics, such as cephalosporin C and the intermediate 7-aminocephalosporanic acid with ≥98% conversion and 91-93% product yield. |
| |
Keywords: | 7-Aminocephalosporanic acid Acetyl esterase Antibiotics Cephalosporin-C Orange peel |
本文献已被 InformaWorld 等数据库收录! |
|