Effect ofTrichoderma species on growth of Fusariumproliferatiom and production of fumonisins,fusaproliferin and beauvericin |
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| Authors: | F. Rojo M. Ferez M. Reynoso A. Torres S. Chulze |
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| Affiliation: | (1) Departamento de Microbiologia e Inmunologia, Facultad de Ciencias Exactas, Fisico-Quimicas y Naturales, Universidad Nacional de Rio Cuarto, Ruta 36 Km 601 (5800) Rio Cuarto, Córdoba, Argentina;(2) Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET), Rivadavia 1917, Buenos Aires, Argentina;(3) Departamento de Microbiologia e Inmunologia, Facultad de Ciencias Exactas, Fco-Qca y Naturales, Universidad Nacional de Rio Cuarto, Rutas 36 Km 601 (5800) Rio Cuarto, Córdoba, Argentina |
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| Abstract: | Trichoderma species has been suggested as potential biocontrol agent forFusarium verticillioides on maize. In this cereal,F. verticillioides and F. proliferatum contributed to fumonisin accumulation. In addition,F. proliferatum could produce beauvericin and fusaproliferin. The aim of this work was to evaluate the effect ofTrichoderma spp. on growth and fumonisin B1 fusaproliferin and beauvericin production byF. proliferatum. Dual cultures of F.proliferatum andT. harzianum ITEM 3636 andT. longibrachiatum ITEM 3635 on maize meal agar at 0.995 aw were done. The effect ofTrichoderma spp. on the lineal growth ofF. proliferatum was determined. The effect ofTrichoderma species on fumonisin B1, fusaproliferin and beauvericin production byF. proliferatum was determined on co-inoculated maize kernels by HPLC.T. harzianum suppressedF. proliferatum growth once contact between the colonies occurred.T. longibrachiatum showed a less antagonistic effect againstF. proliferatum. A reduction on fumonisin B1 production of 98% and 88% was observed in the co-incubation ofF. proliferatum withT. harzianum andT. longibrachiatum, respectively. The decrease of FB1 production was significant even in maize kernels on whichF. proliferatum had been growing 7 days prior to the addition ofTrichoderma spp. The concentration of beauvericin and fusaproliferin produced during 30 days coincubation ofF. proliferatum with bothTrichoderma spp. did not differ to those produced byF. proliferatum alone. These mycotoxins might enter the food chain causing so far unknown consequences to the health of domestic animals and humans. For this reason it is important, when a potential biocontrol agent is under study, to test the effect on the fungal growth and on the putative mycotoxin produced. Part of the information was presented at the Mycotoxin Prevention Cluster Dissemination Day and Mycoglobe Launch Conference, Brussels, Belgium, Oct 20–21, 2004 Financial support: Agenda Córdoba Ciencia, grant No 0279–000431/00 |
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| Keywords: | Trichoderma spp. Fusarium proliferatum fumonisin B1 fusaproliferin beauvericin |
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