| 实时荧光定量PCR技术的研究进展与应用 |
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| 引用本文： | 钟江华,张光萍,柳小英.实时荧光定量PCR技术的研究进展与应用[J].氨基酸和生物资源,2011,33(2):68-72. |
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| 作者姓名： | 钟江华 张光萍 柳小英 |
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| 作者单位： | 武汉大学后勤集团,武汉,430072;武汉大学后勤集团,武汉,430072;武汉大学后勤集团,武汉,430072 |
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| 摘 要： | 实时荧光定量PCR技术(real-time fluorescent quantitative PCR,FQ-PCR)以其特异性强、灵敏度高、重复性好、定量准确、自动化程度高、速度快、全封闭反应等优点在人类和动物疾病的快速检测、食品安全检测、定量分析、基因分型、基因表达研究、以及疫苗效力测定中成为分子生物学研究的重要工具...
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| 关 键 词： | 荧光定量 PCR 原理 应用 |
Development of Real-Time Fluorescent Quantitative PCR and Its Application |
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| ZHONG Jiang-hua,ZHANG Guang-ping,LIU Xiao-ying.Development of Real-Time Fluorescent Quantitative PCR and Its Application[J].Amino Acids & Biotic Resources,2011,33(2):68-72. |
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| Authors: | ZHONG Jiang-hua ZHANG Guang-ping LIU Xiao-ying |
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| Institution: | ZHONG Jiang-hua,ZHANG Guang-ping,LIU Xiao-ying(Logistics Group,Wuhan University,Wuhan 430072,China) |
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| Abstract: | Real-time quantitative PCR becomes the important tool in the molecular biology research in human and animal diseases,food safety detection,quantitative analysis,gene expression,and determination of vaccine effectiveness.The background,principle,type,technology,the experimental conditions and the main influence factors and its application are reviewed. |
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| Keywords: | PCR |
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