| Viili中胞外多糖的分离纯化及对秀丽线虫寿命的影响 |
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| 引用本文： | 王昌禄,韩晓梅,陈勉华,王玉荣,李风娟,韩洪杰,罗成.Viili中胞外多糖的分离纯化及对秀丽线虫寿命的影响[J].氨基酸和生物资源,2011,33(2):64-67. |
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| 作者姓名： | 王昌禄 韩晓梅 陈勉华 王玉荣 李风娟 韩洪杰 罗成 |
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| 作者单位： | 食品营养与安全教育部重点实验室,天津科技大学,食品工程与生物技术学院,天津,300457;食品营养与安全教育部重点实验室,天津科技大学,食品工程与生物技术学院,天津,300457;分子与细胞生物学研究所,塔图大学,塔图,51010,爱沙尼亚 |
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| 摘 要： | 采用Sevage法脱蛋白、乙醇沉淀、DEAE-纤维素离子交换柱层析和Sephadex G-200分子筛层析法,从Viili中分离纯化得到Viili中乳酸菌胞外多糖(EPS).采用秀丽线虫进行寿命实验,观察Viili EPS延长秀丽线虫寿命的效果,在20℃下,饲喂Viili EPS 200 mg·L-1组秀丽线虫的平均寿...
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| 关 键 词： | 胞外多糖 纯化 寿命 秀丽线虫 Viili |
Isolation,Purification of Extracellular Polysaccharide from Viili and Its Effect on the Lifespan of Caenorhabditis elegans |
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| WANG Chang-lu,HAN Xiao-mei,CHEN Mian-hua,WANG Yu-rong,LI Feng-juan,HAN Hong-jie,LUO Cheng.Isolation,Purification of Extracellular Polysaccharide from Viili and Its Effect on the Lifespan of Caenorhabditis elegans[J].Amino Acids & Biotic Resources,2011,33(2):64-67. |
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| Authors: | WANG Chang-lu HAN Xiao-mei CHEN Mian-hua WANG Yu-rong LI Feng-juan HAN Hong-jie LUO Cheng |
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| Institution: | WANG Chang-lu1,HAN Xiao-mei1,CHEN Mian-hua1,WANG Yu-rong1,LI Feng-juan1,HAN Hong-jie1,LUO Cheng1,2(1.Key Laboratory of Food Nutrition and Safety of Ministry of Education,College of Food Engineering and Biotechnology,Tianjin University of Science & Technolgy,Tianjin 300457,China,2.Dept.of Biotechnology,University of Tartu,Tartu 51010,Estonia) |
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| Abstract: | The extracelluar polysaccharide(EPS) from Viili was obtained by Sevage method,ethanol deposition,the DEAE gel chromatography column and Sephadex G-200 chromatography column.Then the effect of Viili EPS on longevity of Caenorhabditis elegans was detected by lifespan test.At 20℃,the mean lifespan of C.elegans was 17.13±0.53 d with Viili EPS 200 μg·mL-1.Compared with the control,the Viili EPS-treated groups showed significant increase in the mean lifespan of C.elegans(p < 0.001). |
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| Keywords: | Viili |
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