| 高效液相色谱法测定黄瓜皮中绿原酸含量 |
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| 引用本文： | 李利华.高效液相色谱法测定黄瓜皮中绿原酸含量[J].氨基酸和生物资源,2011,33(2):73-75. |
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| 作者姓名： | 李利华 |
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| 作者单位： | 陕西理工学院,化学与环境科学学院,陕西,汉中,723001 |
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| 摘 要： | 目的:建立高效液相色谱法测定黄瓜皮中绿原酸含量的方法.方法:采用Zorbax Eclipse XDB-C18色谱柱(150 ×4.6 nm,5 μm);流动相:甲醇- 0.4%磷酸溶液(25∶75);流速:1.0 ml·min-1;检测波长:326nm;柱温:30℃.结果:绿原酸的线性范围为0.0111～0.1332 ...
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| 关 键 词： | 高效液相色谱法 黄瓜皮 绿原酸 |
Determination of Chlorogenic Acid in Cucumber Skin by HPLC |
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| LI Li-hua.Determination of Chlorogenic Acid in Cucumber Skin by HPLC[J].Amino Acids & Biotic Resources,2011,33(2):73-75. |
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| Authors: | LI Li-hua |
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| Institution: | LI Li-hua(College of Chemical and Environmental Sciences,Shaanxi University of Technology,Hanzhong 723001,China) |
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| Abstract: | To establish an HPLC method for determination of chlorogenic acid in cucumber skin,Zorbax Eclipse XDB-C18 was adopted.The mobile phases consisted of CH3OH-0.04% H3PO4(25∶75) at a flow rate of 1.0 mL·min-1,the detection wavelength was set at 326nm,and column temperature was 30 ℃.The linear range of chlorogenic acid was 0.0111～0.1332mg·mL-1(r= 0.9992).The average recoveries(n=5) was 99.97% and RSD was 2.07%.The contents of chlorogenic acid in cucumber skin were more than 8.10 mg·g-1.The method was simple,rapid and accurate with a good reproducibility,and could be applied to determine of chlorogenic acid in cucumber skin. |
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| Keywords: | HPLC cucumber skin chlorogenic acid |
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