Functional expression,purification, and antimicrobial activity of a novel antimicrobial peptide MLH in Escherichia coli |
| |
Authors: | Guo-Li Gong Yuan Wei Zhong-Zhong Wang |
| |
Affiliation: | 1. College of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an, China;2. Xi’an Microbiology and Pharmaceutical Engineering Laboratory, Xi’an, China |
| |
Abstract: | In this study, a novel heterozygous antimicrobial peptide MLH was synthesized, expressed, purified, and characterized. The peptide Md-cec-LL-37_Hp (MLH) was selected through bioinformatic analysis using musca domestica antimicrobial peptide (Cec-Med), human antimicrobial peptide LL-37, and helicobacter pylori antimicrobial peptide (Hp) as parent peptides. The target gene was synthesized by overlap extension PCR (SOE-PCR) and connected to the expression vector pET-32a (+), and the recombinant plasmid pET-32a-MLH was transformed to Escherichia coli for constructing pET-32a-MLH/BL21 (DE3). Isopropyl β-D-thiogalactoside (IPTG) was used to induce protein expression, and SDS-PAGE and western blot were adopted to test the target protein. And fermentation condition was optimized to get the mass expression of the fusion protein. The Ni2+ affinity chromatographic column was used to purify. Active heterozygous peptide was obtained after renaturation. Finally, the activity of the heterozygous antimicrobial peptide was identified. The fusion peptide showed significant antimicrobial effect on both E. coli and Staphylococcus aureus. |
| |
Keywords: | Antimicrobial activity Escherichia coli fusion expression heterozygous antimicrobial peptide |
|
|