Production and partial characterization of β-1,3-glucanase obtained from Rhodotorula oryzicola |
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| Authors: | Mona Liza Santana Elinalva Maciel Paulo José Ailton Bispo Amanda Reges de Sena |
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| Institution: | 1. Health Department, State University of Feira de Santana (UEFS), Feira de Santana, Brazil;2. Department of Biological Sciences, LAMASP, State University of Feira de Santana (UEFS), Feira de Santana, Brazil;3. Department of Technology, State University of Feira de Santana (UEFS), Feira de Santana, Brazil;4. Microbiology Laboratory, Federal Institute of Education, Science and Technology of Pernambuco, Barreiros, PE, Brazil |
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| Abstract: | The current study aims to assess the kinetics of population growth of Rhodotorula oryzicola and the production of β-1,3-glucanase (EC 3.2.1.39) enzyme by this yeast. It also aims to obtain the optimum conditions of β-1,3-glucanase enzymatic activity by varying the pH as well as to study the enzyme thermostability. R. oryzicola population doubled within 12?hr. During this period, 9.26 generations were obtained, with 1?hr and 29?min of interval from one generation to the other, with specific growth rate (µ) of 0.15 (hr?1). The entire microorganism growth process was monitored during β-1,3-glucanases production, and the maximum value was obtained in the stationary phase in the 48-hr fermentation period. pH and temperature optimum values were 4.7 and 96°C, respectively. The enzyme maintained 88% of its activity when submitted to the temperature of 90°C for an incubation period of 1?hr. The results show that the enzyme can be used in industrial processes that require high temperatures and acidic pH. |
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| Keywords: | Doehlert experimental planning kinetics Rhodotorula oryzicola β-1 3-glucanases |
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