Plant cell suspension culture in a bench-scale fermenter with a newly designed membrane stirrer for bubble-free aeration |
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Authors: | C Böhme M-B Schröder H Jung-Heiliger J Lehmann |
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Institution: | AG Zellkulturtechnik, Technische Fakult?t, Universit?t Bielefeld, Postfach 100131, D-33501 Bielefeld, Germany Tel. (49) 521 106 6322; Fax (49) 521 106 6328 e-mail: cbo@zellkult.techfak.uni-bielefeld.de, DE Institut für Lebensmittelchemie, TU Braunschweig, Schleinitzstr. 20, D-38106 Braunschweig, Germany, DE Celler Pflanzen- und Gewebelabor GmbH, Postfach 1246, D-29202 Celle, Germany, DE
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Abstract: | In this paper, tests of an optimized membrane-stirrer geometry for bubble-free aeration of a plant cell suspension culture
are described. Cell attachment and clogging of a previously described system Piehl et al. (1988) Appl Microbiol Biotechnol
29:456–461] led to the development of a new stirrer. The volumetric oxygen transfer capacity has been measured in aqueous
medium. The mass transfer coefficient, k
l
a, was 3.75 h−1 at 25 °C and at a stirrer speed of 34 rpm. The overall oxygen transfer capacity was investigated with a suspension culture
of Aesculus hippocastanum. It was shown that the oxygen mass transfer was sufficient even at the maximum biomass of 10–12 g dry weight/l, which was
obtained by using this system. Furthermore, special attention was given to medium components like C and N sources, to avoid
growth limitation due to a shortage of nutrients.
Received: 22 October 1996 / Revised version: 11 March 1997 / Accepted: 14 March 1997 |
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