Cultivation of the sapropelic ciliate Plagiopyla nasuta Stein and isolation of the endosymbiont Methanobacterium formicicum |
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Authors: | Nico K Goosen Anja M C Horemans Silvia J W Hillebrand Claudius K Stumm Godfried D Vogels |
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Institution: | (1) Department of Microbiology, Faculty of Science, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands |
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Abstract: | The sapropelic ciliate Plagiopyla nasuta was isolated and cultured in monoculture. Optimal conditions for growth were: 15–20°C, pH about 7, and about 2% of oxygen in the headspace. Cultures of P. nasuta produced methane. Epifluorescence microscopy revealed the presence of methanogenic bacteria as endosymbionts. An endosymbiont of the ciliate was isolated and identified as Methanobacterium formicicum. In the ciliate cell these methanogens were found to be closely associated with microbody-like organelles. No mitochondria could be detected. |
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Keywords: | Ciliate culture Endosymbiosis Methanobacterium formicicum Methanogenic bacteria Plagiopyla nasuta Stein Sapropelic protozoa |
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