A unique deletion in pBR322 DNA caused by insertion of poly(dA) · poly(dT) |
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Authors: | Emeka V. Ikpeazu Manuel S. Valenzuela |
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Abstract: | A unique deletion covering around 43% of the pBR322 genome was found after attempting to insert 100 or 200 bp poly(dA) · poly(dT) into the EcoRV site of pBR322 DNA. This result was not observed if an equivalent size heterologous DNA or a larger poly(dA) · poly(dT) fragment of 10–20,000 bp was introduced at the same site. DNA sequencing analysis at the junctions suggests that a specific intramolecular pairing may be involved in the formation of this deletion mutant. |
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