PqqC/D,which converts a biosynthetic intermediate to pyrroloquinoline quinone |
| |
Authors: | Toyama Hirohide Fukumoto Hiroyuki Saeki Megumi Matsushita Kazunobu Adachi Osao Lidstrom Mary E |
| |
Institution: | Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 753-8515, Yamaguchi, Japan. toyama@agr.yamaguchi-u.ac.jp |
| |
Abstract: | PqqC/D was purified from Escherichia coli transformant. The purified enzyme converted an intermediate that accumulated in a pqqC mutant of Methylobacterium extorquens AM1 to PQQ. The reaction did not show any dependence of NAD(P)H that was observed in the crude extract before purification. PqqC/D reacted with the intermediate stoichiometrically, but not catalytically. When partially purified proteins from the crude extract of E. coli were added to the reaction mixture, the rate of PQQ production increased dependent on the amount of NADPH added and the total amount of PQQ produced increased. |
| |
Keywords: | Pyrroloquinoline quinone PQQ Methylotrophs Biosynthesis |
本文献已被 ScienceDirect PubMed 等数据库收录! |