Paenibacillus taohuashanense sp. nov., a nitrogen-fixing species isolated from rhizosphere soil of the root of Caragana kansuensis Pojark |
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Authors: | Jian-Bo Xie Li-Hong Zhang Yu-Guang Zhou Hong-Can Liu San-Feng Chen |
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Affiliation: | 1. State Key Laboratory for Agrobiotechnology, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, 100193, People’s Republic of China 2. China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, 100101, People’s Republic of China
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Abstract: | A nitrogen-fixing bacterium, designated strain gs65T, was isolated from a rhizosphere soil sample of Caragana kansuensis Pojark. Phylogenetic analysis based on a fragment of the nifH gene and the full-length 16S rRNA gene sequence revealed that strain gs65T is a member of the genus Paenibacillus. High levels of 16S rRNA gene similarity were found between strain gs65T and Paenibacillus borealis DSM 13188T (97.5 %), Paenibacillus odorifer ATCC BAA-93T (97.3 %), Paenibacillus durus DSM 1735T (97.0 %) and Paenibacillus sophorae DSM23020T (96.9 %). Levels of 16S rRNA gene sequence similarity between strain gs65T and the type strains of other recognized members of the genus Paenibacillus were below 97.0 %. Levels of DNA–DNA relatedness between strain gs65T and P. borealis DSM 13188T, P. odorifer ATCC BAA-93T (97.3 %), P. durus DSM 1735T and P. sophorae DSM23020T were 35.9, 38.0, 34.2 and 35.5 % respectively. The DNA G+C content of strain gs65T was determined to be 51.6 mol%. The major fatty acids were found to be iso-C14:0, anteiso-C15:0 and iso-C16:0. On the basis of its phenotypic characteristics and levels of DNA–DNA hybridization, strain gs65T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus taohuashanense sp. nov. is proposed. The type strain is gs65T (=CGMCC 1.12175T = DSM 25809T). |
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