Cytoskeletal structures,ultrastructural characteristics and the capsule of the basidiomycetous yeast <Emphasis Type="Italic">Cryptococcus laurentii</Emphasis> |
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Authors: | Marek David Miroslav Gabriel Marie Kopecká |
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Institution: | (1) Department of Biology, Faculty of Medicine, Masaryk University, Tomešova 12, Brno, 602 00, Czech Republic |
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Abstract: | The cytoskeleton, capsule and cell ultrastructure were studied during the cell cycle of Cryptococcus laurentii. In an encapsulated strain, cytoplasmic microtubules and a mitotic spindle were detected. Mitosis was preceded by migration
of the nucleus into the bud. F-actin failed to be visualised by rhodamine-phalloidin (RhPh) in encapsulated cells and therefore
an acapsular strain was used. The following actin structures were found: actin dots, actin cables and cytokinetic ring. Ultrastructural
studies showed the presence of a nucleus in the bud before mitosis. A collar-shaped structure was seen at the base of bud
emergence. A lamellar cell wall and a rough outer surface of the cells were detected. Cytoskeletal structures found in C. laurentii are similar to those in Cryptococcus neoformans, which is a serious human pathogen. |
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Keywords: | Capsule Cell cycle Cryptococcus laurentii Cytoskeleton Morphology Ultrastructure |
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