Nuclear DNA variability withinPisum sativum (Leguminosae): Cytophotometric analyses |
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| Authors: | Andrea Cavallini Lucia Natali |
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| Institution: | (1) Sezione di Genetica, Dipartimento di Biologia delle Piante Agrarie della Università di Pisa, via Matteotti 1/B, I-56100 Pisa, Italy |
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| Abstract: | The cultivars or experimental lines ofPisum sativum were analyzed cytophotometrically for nuclear DNA content of early prophases after ethanol-acetic acid fixation. Wide variability was found (from 3.93 to 5.07pg per haploid nucleus). This result was confirmed by the cytophotometric analysis of interphase nuclei isolated from leaf tissues fixed in formalin. Analysis of interphase nuclei at different thresholds of optical density showed that certain chromatin fractions are involved in the variations. |
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| Keywords: | Angiosperms Leguminosae Pisum sativum Cytophotometry nuclear DNA content DNA variability |
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