An IR-investigation of the helix-coil conversion of poly U |
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Authors: | D Bode M Heinecke U Schernau |
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Affiliation: | Institut für Physikalische Chemie der Universität Freiburg, 78 Freiburg, Hebelstrasse 38, West-Germany. |
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Abstract: | In the presence of two or polyvalent cations, e. g., Mg2+, protonated ethylenediamine, protonated spermidine, and protonated spermine, poly U forms an ordered structure in aqueous solution. At elevated temperatures, the secondary structure is lost by a cooperative process. The melting temperature Tm rises with the size of the cations. Using IR-spectroscopy, we could show that the secondary structure of poly U is stabilized by hydrogen bonds. Both carbonyl groups of the uracil are involved in the hydrogen bonding. This experimental result enables us to choose the right arrangement for UU base pairing. |
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