Evidence for amphithallism and broad geographical hybridization potential among Agaricus subrufescens isolates from Brazil,France, and Thailand |
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| Institution: | 1. Institute of Excellence in Fungal Research, and School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand;2. INRA, UR1264 MycSA, Mycologie et sécurité des aliments, CS 20032, 33883 Villenave d’Ornon Cedex, France;3. Aurora University, Biology Department, 347 S. Gladstone Ave., Aurora, IL 60506-4892, USA;4. Sylvan Biosciences, 198 Nolte Drive, Kittanning, PA 16201, USA;1. Colegio de Postgraduados (CP), Campus Puebla, Biotecnología de Hongos Comestibles, Funcionales y Medicinales & Recursos Fitogenéticos, Apartado Postal 129, 72001, Puebla, Mexico;2. Universidad Nacional Autónoma de México (UNAM), Departamento de Alimentos y Biotecnología, Facultad de Química, Ciudad Universitaria, Coyoacán, CDMX, 04510, Mexico;1. Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, São José dos Campos, SP 12244-000, Brazil;2. Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP 14040-903, Brazil;1. Department of Biosciences, University of Helsinki, Helsinki, FI-00014, Finland;2. Herbarium, University of Turku, Turku, FI-20014, Finland;3. Botanical Museum, Finnish Museum of Natural History, University of Helsinki, Helsinki, FI-00014, Finland;1. Departamento de Biotecnología, Universidad Autónoma Metropolitana –Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, Iztapalapa, 09340 México D.F., Mexico;2. Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana –Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, Iztapalapa, 09340 México D.F., Mexico;1. Agricultural University of Athens, Laboratory of General and Agricultural Microbiology, Iera Odos 75, 11855 Athens, Greece;2. Democritus University of Thrace, Department of Environmental Engineering, Laboratory of Wastewater Management and Treatment Technologies, Vas. Sofias 12, 67100 Xanthi, Greece;3. Università di Palermo, Department of Agricultural and Forest Sciences, Viale delle Scienze, 11, 90128 Palermo, Italy;4. John Innes Centre, Department of Disease and Stress Biology, Colney Lane, Norwich NR4 7UH, UK;5. National and Kapodistrian University of Athens, Department of Genetics and Biotechnology, Faculty of Biology, Panepistemiopolis, Athens 15701, Greece |
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| Abstract: | Agaricus subrufescens is a cultivated edible and medicinal mushroom. Its known geographical distribution encompasses the Americas, Europe, Oceania, and Asia. The objective of this study was to assess mating compatibility and interfertility of strains originating from Brazil, France, and Thailand. Progeny of each strain were analyed with codominant molecular markers. Multilocus genotype tests revealed that the three strains were amphithallic with percentages of heterokaryotic single spore progenies of 75 % for the Thai strain and around 40 % for the Brazilian and French strains. In mating tests A. subrufescens had a multiallelic unifactorial system of sexual incompatibility. The three parent strains were interfertile based on experimental pairings of single-spore isolates, the recovery of hybrid heterokaryons from compatible matings, and the ability of hybrids to produce mushrooms and fertile spores. This biological approach supports the inclusion of the European strains within the species and the extension of the geographical distribution range to Asia. Our data should help to develop breeding strategies and to better manage and exploit the diversity existing in A. subrufescens. |
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| Keywords: | Interfertility Life cycle Meiosporogenesis Postmeiotic mitosis Pseudohomothallism |
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