Comparison of HPV genotypes and viral load between different sites of genital tract: The significance for cervical cancer screening |
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| Institution: | 1. Department of Cancer Epidemiology, Cancer Institute/Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;2. Merck Sharp and Dohme, Whitehouse Station, NJ, USA;3. University of Miami Miller School of Medicine, Miami, FL, USA;4. Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, USA;1. Department of Microbiology, University Hospital of Vigo, Vigo, Pontevedra, Spain;2. Department of Pathology, University Hospital of Vigo, Vigo, Pontevedra, Spain;3. Department of Gynecology, University Hospital of Vigo, Vigo, Pontevedra, Spain;4. Department of Virology, University Hospital of Asturias, Oviedo, Spain;1. Department of Human Anatomy and Experimental Oncology, Research Institute for Health Sciences and Technology, Faculty of Medicine and Pharmacy, University of Mons, 6 Ave du Champ de Mars, B-7000 Mons, Belgium;2. Department of Pathology, C.H.U. – SART TILMAN, University of Liège, 4000 Liège, Belgium;3. Laboratory of Oncology and Experimental Surgery, Jules Bordet Institute, Université Libre de Bruxelles, 1000 Brussels, Belgium;4. Department of Oto-Rhino-Laryngology, Faculty of Medicine, Free University of Brussels (ULB), CHU St-Pierre, Rue Haute, 322, B-1000 Brussels, Belgium;1. Faculty of Health Sciences, School of Medicine, Universidad Peruana de Ciencias Aplicadas-UPC, Lima, Peru;2. Instituto de Investigación Nutricional, Lima, Peru;3. Instituto de Investigación de Enfermedades Infecciosas, Cajamarca, Peru;4. Liga de Lucha Contra el Cáncer-Cajamarca, Peru;5. Centre de Biotecnologia Molecular (CEBIM), Departament d''Enginyeria Química, ETSEIB, Universitat Politècnica de Catalunya (UPC) Barcelona Tech, Spain;6. Fundación Clinic, IDIBAPS, Hospital Clinic i Provincial de Barcelona, Spain;7. Barcelona Centre for International Health Research (CRESIB, Hospital Clínic-Universitat de Barcelona), Barcelona, Spain;1. Molecular Biology Laboratory, Center for Translational Research in Oncology, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil;2. Department of Cancer Epidemiology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA;3. Infections and Cancer Biology Group, International Agency for Cancer Research (IARC), Lyon, France;4. Ludwig Institute for Cancer Research, São Paulo branch, São Paulo, Brazil;5. Centro de Referência e Treinamento DST/Aids, São Paulo, Brazil;6. Centro de Investigación en Salud Poblacional, Instituto Nacional de Salud Pública, Cuernavaca, Mexico;7. Department of Radiology and Oncology, School of Medicine, University of São Paulo, São Paulo, Brazil;1. Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico;2. Colegio de la Frontera Norte, Piedras Negras, Coahuila, Mexico |
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| Abstract: | ObjectivesTo compare the consistency of HPV genotype and viral loads among different sites within the female genital tract, and to correlate these with clinical outcomes.Methods2646 previously unscreened rural women were enrolled in this population-based, cross-sectional study between May 2006 and April 2007. Physician-collected samples from lower vagina, upper vagina, cervix, and one self-collected sample were taken from each woman. Viral load was assessed by HC2 using the relative light unit/cutoff ratio (RLU/CO), and HPV genotyping was tested by Linear Array.ResultsThe low risk HPV positive rate was highest in lower vagina samples and lowest in cervix samples. Overall kappa values of high risk HPV types between various anatomic sampling sites showed substantial or almost perfect agreement among women with normal pathology, CIN1, and CIN2+. In the CIN2+ population, high risk HPV viral load for cervix samples (557.25 RLU/CO) were much higher than upper vagina samples (96.43 RLU/CO, P < 0.001), lower vagina samples (36.51 RLU/CO, P < 0.001), and self-collected (206.83 RLU/CO, P = 0.003) samples.ConclusionsAlthough the distribution of high risk HPV genotypes was fairly equivalent across different genital sites, particularly for CIN2+ lesions, viral loads were largely variable. The findings may affect the cervical cancer screening methods using self-collected samples, particularly in resource-challenged areas. |
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| Keywords: | Human papillomavirus Genotype Viral load Intraepithelial neoplasia Genital tract |
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