MicroRNA-451 suppresses tumor cell growth by down-regulating IL6R gene expression |
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| Institution: | 1. Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China;2. Department of Medical Genetics, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China;3. Institute for Experimental Pathology and Faculty of Medicine, University of Iceland, Keldur, Reykjavik, Iceland;1. Department of Clinical Nursing, Division of Health Sciences, Graduate Course of Nursing Science, Kanazawa University, Kanazawa, Japan;2. Faculty of Nursing, Osaka Medical College, Osaka, Japan;3. Department of Quantum Medical Technology, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan;4. Department of Gerontological Nursing/Wound Care Management, Division of Health Science and Nursing, Graduate School of Medicine, University of Tokyo, Japan;5. Department of Clinical Nursing, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa, Ishikawa 920-0942, Japan;1. Consiglio per la Ricerca e la sperimentazione in Agricoltura, Viale Piacenza 29, 26900 Lodi, Italy;2. Consiglio per la Ricerca e la sperimentazione in Agricoltura, Via Canton 14, 37051 Bovolone, Verona, Italy;3. Consiglio per la Ricerca e la sperimentazione in Agricoltura, Via di Corticella 133, 40128 Bologna, Italy;4. Università di Milano, Facoltà di Medicina Veterinaria, Via Celoria 10, 20133 Milano, Italy;1. College of Computer Science, Chongqing University, Chongqing, 400044, China;2. School of Electronic and Information Engineering, Southwest University, Chongqing, 400715, China;3. College of Computer Science, Chongqing University of Posts and Telecommunications, Chongqing, 400065, China |
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| Abstract: | The miR-451 was found to be frequently down-regulated in tumors, indicating that miR-451 could play an important role in carcinogenesis. This study uncovered the mechanism by which the miR-451 functions as a tumor suppressor. The target genes of miR-451 were determined using target gene prediction softwares. Then the miR-451 mimics were introduced into RKO and Hela cells respectively. The proliferation and invasion of cells were monitored by MTT, cell cycle and in vitro extracellular matrix invasion assays. Also the angiogenesis of HUVEC cells transfected with miR-451 mimics was examined. Subsequently, IL6R, a predicted target gene of miR-451, was studied by real time PCR, Western blotting, and siRNA technologies. The mRNA and protein levels of IL6R gene were found to be down-regulated in the RKO and Hela cells transfected with miR-451 mimics. Consequently, the cell proliferation was inhibited. Also, the invasion of RKO cells was suppressed. Furthermore, the angiogenesis of HUVEC cells transfected with miR-451 mimics was assayed and the decreased angiogenic ability was detected compared to the controls. All these results were validated by IL6R siRNA experiments. The IL6R gene is a target gene of miR-451. The miR-451 behaves as a tumor suppressor, probably by targeting the IL6R pathway. |
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| Keywords: | miR-451 Tumor suppressor IL6R Down-regulating |
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