Studies of the identity of the unknown in protein hydrolysates |
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| Authors: | W K Paik S Kim |
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| Institution: | 1. Department of Physiological Chemistry and Nutrition Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;2. Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;3. Department of Enteroviruses, National Institute of Health, Musashimurayama-shi, Tokyo 190-12, Japan |
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| Abstract: | DNA synthesis in the adenovirus DNA replication complex, containing host DNA polymerases-α and -γ, was inhibited completely by aphidicolin and by 2′,3′-dideoxythymidine triphosphate (ddTTP). Double reciprocal plots of DNA polymerase activity in the replication complex against each dNTP gave a straight line although the complex contained two species of DNA polymerase. Inhibition by aphidicolin of DNA polymerase activity was competitive with dTTP but that of purified DNA polymerase-α isolated from adenovirus infected KB cells was competitive with dCTP. The above results suggest that DNA polymerases-α and -γ are integrated in the replication complex to behave as a single enzyme. |
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