首页 | 本学科首页   官方微博 | 高级检索  
     


Polyunsaturated Fatty Acids Attenuate Diet Induced Obesity and Insulin Resistance,Modulating Mitochondrial Respiratory Uncoupling in Rat Skeletal Muscle
Authors:Gina Cavaliere  Giovanna Trinchese  Paolo Bergamo  Chiara De Filippo  Giuseppina Mattace Raso  Giorgio Gifuni  Rosalba Putti  Bottu Heleena Moni  Roberto Berni Canani  Rosaria Meli  Maria Pina Mollica
Affiliation:1. Department of Biology, University of Naples “Federico II”, Napoli, Italy;2. Institute of Food Sciences, CNR-ISA, Avellino, Italy;3. Department of Pharmacy, University of Naples “Federico II”, Napoli, Italy;4. Department of Translational Medical Sciences, European Laboratory for Food Induced Diseases, University of Naples “Federico II”, Napoli, Italy;Monash University, AUSTRALIA
Abstract:

Objectives

Omega (ω)-3 polyunsaturated fatty acids (PUFA) are dietary compounds able to attenuate insulin resistance. Anyway, the precise actions of ω-3PUFAs in skeletal muscle are overlooked. We hypothesized that PUFAs, modulating mitochondrial function and efficiency, would ameliorate pro-inflammatory and pro-oxidant signs of nutritionally induced obesity.

Study Design

To this aim, rats were fed a control diet (CD) or isocaloric high fat diets containing either ω-3 PUFA (FD) or lard (LD) for 6 weeks.

Results

FD rats showed lower weight, lipid gain and energy efficiency compared to LD-fed animals, showing higher energy expenditure and O2 consumption/CO2 production. Serum lipid profile and pro-inflammatory parameters in FD-fed animals were reduced compared to LD. Accordingly, FD rats exhibited a higher glucose tolerance revealed by an improved glucose and insulin tolerance tests compared to LD, accompanied by a restoration of insulin signalling in skeletal muscle. PUFAs increased lipid oxidation and reduced energy efficiency in subsarcolemmal mitochondria, and increase AMPK activation, reducing both endoplasmic reticulum and oxidative stress. Increased mitochondrial respiration was related to an increased mitochondriogenesis in FD skeletal muscle, as shown by the increase in PGC1-α and -β.

Conclusions

our data strengthened the association of high dietary ω3-PUFA intake with reduced mitochondrial energy efficiency in the skeletal muscle.
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号