多孔n-HA/PA66支架复合rhBMP-2修复兔桡骨缺损的实验研究

目的 ：研究多孔纳米羟基磷灰石/聚酰胺66（nHA/PA66）骨修复材料作为骨组织工程支架复合基因重组人骨形态发生蛋白2（rhBMP2）后的成骨能力的变化，探讨加速nHA/PA66人工骨与受体骨愈合的方法。方法：选用新西兰大白兔双侧桡骨制作骨缺损模型，将nHA/PA66/rhBMP2复合材料植入左侧骨缺损处，右侧骨缺损以nHA/PA66植入作为实验对照，另做不植入任何材料的骨缺损空白对照。在1、2、4、8、12周各时相点分别进行大体观察、X线照片、组织学切片、免疫组化原位杂交进行检测图象分析。结果：nHA/PA66/BMP2与nHA/PA66组骨缺损均完全修复，而空白对照组骨缺损未见修复；2周时nHA/PA66与nHA/PA66/rhBMP2两组间原位杂交阳性细胞表达有统计学意义（ P<0.05）， 4周时nHA/PA66与nHA/PA66/rhBMP2两组间原位杂交阳性细胞表达无统计学意义（ P>0.05），2周及4周实验和实验对照两组分别与空白对照组比较均无统计学意义（ P>0.05），nHA/PA66/rhBMP2组较nHA/PA66组可加速人工骨/植入体/受体界面骨愈合。 结论：多孔nHA/PA66作为骨组织工程支架复合具有诱导成骨活性的rhBMP2后，增强了早期成骨能力，加速了其与受体骨的愈合。

An Experimental Study on Using the Porous Composite of Nano-hydroxyapatite and Polymide as Scaffold Compound with rhBMP-2 to Repairing Bone Defect on Rabbits

AbstractObjective:To evaluate the ability of the porous nanohydroapatite crystals and polyamide composite (nHA/PA66) as scaffold  compound with rhBMP2 to repairing bone defect, study the methods which fast the artificial bone with host bone healing in vivo.Methods: The animal models of bilaterial radius bone defect created with surgery in the New Zealand white rabbits, and were implanted with nHA/PA66/ rhBMP2 as experimental group, implanted nHA/PA66 as experimental control group, and no implant as blank control group. The effect were observed by gross Xray and histopathlogical examination and in situ hybridization at1, 2, 4, 8, 12W after operation, and use the spss12.0 and OneWayANOVA system to analysis. Results: The defects of experimental group and experimental control group were repair perfectly, The blank control group hadn't repaired after 12weeks, the speed of bonehealing of nHA/PA66/ rhBMP2 group were faster than nHA/PA66 group in the early time. The data of  in situ hybridization analysis had  statistically significant difference between nHA/PA66/ rhBMP2  and nHA/PA66 group in two weeks, but the data had no statistically significant difference between nHA/PA66/ rhBMP2 and nHA/PA66 group in four weeks . Conclusion:The results show porous scaffold of nHA/PA66 compound with rhBMP2 can enhance the ability of the artificial bone with host bone healing in the early time in vivo.