In vitro propagation of Pelecyphora aselliformis Ehrenberg and P. strobiliformis Werdermann (cactaceae) |
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Authors: | Email author" target="_blank">Eugenio?Pérez-Molphe-BalchEmail author Carlos?Antonio?Dávila-Figueroa |
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Institution: | (1) Department of de Química, Universidad Autónoma de Aguascalientes, Edificio 60, Av Universidad 940, 20100 Aguascalientes, Ags, Mexico |
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Abstract: | Summary Development of efficient in vitro propagation, systems for Pelecyphora aselliformis Ehrenberg and P. strobiliformis Werdermann,
two endangered Mexican species of cacti, are described. Multiple shoot formation from areoles of in vitro-germinated plantlets
was achived in two types of explants (apical and transversal) cultured in Murashige and Skoog (MS) basal media supplemented
with 30 or 50 gl−1 sucrose, 10 gl−1 agar and various treatments with cytokins. Shoot production in these proliferation media was evaluated after one (60 d) and
three (180 d) culture cycles. In P. asellifornis 13.7 shoots per explant were produced after the first cycle using apical
explants in medium with 8.8 μM 6-benzylaminopurine (BA) and 30 gl−1 sucrose. In P. strobiliformis the highest proliferation rate (12.4 shoots per explant) was reached using 8.8 μM Ba and 50
gl−1 sucrose with shoot transverse segments as explants. After the third proliferation cycle, 128.1 and 136.3 shoots per explant
were obtained in P. aselliformis and P. strobiliformis, respectively. The shoots were clongated in MS basal medium with 3
gl−1 activated charcoal and rooted in MS basal media indoleacetic acid (2.85 or 5.71 μM) or indolebutyric acid (2.46 or 4.90 μM).
On averge, rooting efficiency was 89% for P. aselliformis and 87% for P. strobiliformis. The survival frequency, of the plants
once transferred, to soil was on average 88%. |
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Keywords: | areole activation cytokinin micropropagation tissue culture |
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