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Binding sites of sorbed uranyl ion in the cell wall of Mycobacterium smegmatis
Authors:Yves Andres  H.John MacCordick  Jean-Claude Hubert
Affiliation:Laboratoire de Microbiologie, URA CNRS D-1481, UniversitéLouis Pasteur, Institut Le Bel, F-67000 Strasbourg, France; Laboratoire de Chimie Nucléaire, CRN, B.P. 20, F-67037 Strasbourg Cedex 2, France
Abstract:Abstract A study of cell-wall site interaction of uranyl ion adsorbed by non-proliferative suspensions of Mycobacterium smegmatis at pH 1 has been carried out using extracts of arabinogalactan-peptidoglycan and phospholipids obtained from whole cells treated under sorption conditions. Evidence for binding of UO22+ by constituent P-lipids was provided by comparative 31P-NMR and IR spectroscopic measurements of the isolated wall fractions and of the model complex uranyl-phosphatidyl inositol.
Keywords:Uranyl ion    Binding sites    Phospholipid    Peptidoglycan    Mycobacterium smegmatis
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