Rapid and sensitive identification of pathogenic and apathogenic Bacillus anthracis by real-time PCR |
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Authors: | Ellerbrok Heinz Nattermann Herbert Ozel Muhsin Beutin Lothar Appel Bernd Pauli Georg |
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Affiliation: | Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany. ellerbrokh@rki.de |
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Abstract: | Bacillus anthracis spores have been shown to be an efficient biological weapon and their recent use in bioterrorist attacks has demonstrated the need for rapid and specific diagnostics. A TaqMan real-time PCR for identification of B. anthracis was developed, based on the two plasmids, pX01 and pX02, both of which are necessary for pathogenicity, as well as on the chromosomally encoded rpoB gene. Bacteria picked from colonies or pelleted from liquid cultures were directly inoculated into the PCR mix, thus avoiding time-consuming DNA preparation and minimizing handling risks. B. anthracis spores were cultivated for a few hours in enrichment broth before PCR analysis, or used directly for real-time PCR, thus allowing to confirm or exclude potential attacks approximately 2-3 h after the material has arrived in the laboratory. |
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Keywords: | Anthrax Bacillus cereus group real-time PCR spores bioterrorist attack Bacillus anthracis |
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