Production of Chitinase and its Optimization from a Novel Isolate Serratia marcescens XJ-01 |
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Authors: | Jin-Lan Xia Jing Xiong Rui-Yong Zhang Ke-Ke Liu Bin Huang Zhen-Yuan Nie |
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Affiliation: | (1) School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China;(2) Key Laboratory of Biometallurgy of the Ministry of the Education of China, Central South University, Changsha, 410083, China |
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Abstract: | Production of chitinase from bacteria has distinct advantages over fungi, due to the formation of mycelia of fungi in the later phase of fermentation. A novel chitinase-producing bacterial strain XJ-01 was isolated from the Yulu fishing field of Changsha, Hunan province, China, by enrichment and spread-plate technique, sequentially. Physicochemical characterization and 16S rRNA sequencing revealed that strain XJ-01 belongs to Serratia marcescens. By optimizing the fermentation condition based on L9(34) orthogonal experimental design, a maximal chitinase activity up to 15.36 U/ml was attained by that stain under the condition: 0.5% (NH4)2SO4 as the nitrogen source, 0.75% colloidal chitin as the carbon source, temperature of 32°C, time of 32 h and pH 8.0. |
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Keywords: | Serratia marcescens Chitinase Isolation and characterization Fermentation optimization |
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