Glucose biosensor based on titanium dioxide-multiwall carbon nanotubes-chitosan composite and functionalized gold nanoparticles |
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Authors: | Meihe Zhang Ruo Yuan Yaqin Chai Wenjuan Li Huaan Zhong Cun Wang |
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Affiliation: | (1) Key Education Ministry Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China; |
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Abstract: | In this paper, a new glucose biosensor was prepared. At first, Prussian blue (PB) was electrodeposited on a glassy carbon electrode (GCE) modified by titanium dioxide-multiwall carbon nanotubes-chitosan (TiO2-MWNTs-CS) composite, and then gold nanoparticles functionalized by poly(diallyldimethylammonium chloride) (PDDA-Au) were adsorbed on the PB film. Finally, the negatively charged glucose oxidase (GOD) was self-assembled on to the positively charged PDDA-Au. The electrochemical performances of the modified electrodes had been studied by cyclic voltammetry (CV) and amperometric methods, respectively. In addition, the stepwise fabrication process of the as-prepared biosensor was characterized by scanning electron microscopy. PDDA-Au nanoparticles were characterized by ultraviolet–vis absorption spectroscopy and transmission electron microscopy. Under the optimal conditions, the as-prepared biosensor exhibited a good response performance to glucose with a linear range from 6 μM to 1.2 mM with a detection limit of 0.1 μM glucose (S/N = 3). In addition, this work indicated that TiO2-MWNTs-CS composite and PDDA-Au nanoparticles held great potential for constructing biosensors. |
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