磷酸化蛋白质组研究中的富集策略

蛋白质的磷酸化与去磷酸化过程,调控着包括信号转换、基因表达、细胞周期等诸多细胞过程。因此,对蛋白质磷酸化修饰的分析是蛋白质组研究中的重要内容。但由于磷酸化蛋白的丰度较低,难以用质谱直接检测。为了解决这个问题,改善质谱对磷酸肽的信号响应,需要对磷酸化蛋白质或磷酸肽进行富集。目前主要的富集方法包括免疫沉淀、固相金属离子亲和色谱、金属氧化物/氢氧化物亲和色谱等。

The Enrichment Technique Strategies in Phosphoproteomics Study

In eukaryotes protein phosphorylation is a key event.By reversible protein phosphorylation eukaryotes control many cellular processes including signal transduction,gene expression,the cell cycle etc.The study of phosphoproteomics involves identification of phosphoproteins and phosphopeptides,localization of the exact residues that are phosphorylated and quantitation of phosphorylation.The detection of phosphopeptides remains challenging because of the low abundance of phosphoproteins.The enrichment of phosphoproteins and phosphopeptides is one of the important ways to improve the MS response of phosphopeptide signals.The methods currently available for the enrichment strategies,including using antibodies,immobilized metal affinity chromatography and metal oxide/hydroxide affinity chromatography were summarized in this review.