Combination of multiplex PCR and PCR-denaturing gradient gel electrophoresis for monitoring common sourdough-associated Lactobacillus species |
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| Authors: | Settanni Luca Valmorri Sara van Sinderen Douwe Suzzi Giovanna Paparella Antonello Corsetti Aldo |
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| Affiliation: | Dipartimento di Scienze degli Alimenti, Sezione di Microbiologia Agro-Alimentare ed Ambientale, Università degli Studi di Teramo, V. C. R. Lerici 1, 64023 Mosciano Sant'Angelo, Teramo, Italy. |
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| Abstract: | A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs. |
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