Extracellular β-D-glucosidase of the Penicillium canescens marine fungus |
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Authors: | Yu V Dubrovskaya V V Sova N N Slinkina S D Anastyuk M V Pivkin T N Zvyagintseva |
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Institution: | 1. Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Far Eastern Branch, Vladivostok, 690022, Russia
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Abstract: | Extracellular β-D-glucosidase was isolated in a homogeneous state from the Penicillium canescens marine fungus. According to SDS-electrophoresis, the molecular weight of the enzyme was 64 kDa and the maximal activity was
observed at pH 5.2 and 70°C. Glucosidase catalyzed the hydrolysis of β-glycosidic bonds both in glycosides and in glucose
disaccharides and had transglycosylation activity. The enzyme can be used for the deglycosylation of natural glycosides and
in enzymatic synthesis of new carbohydrate—containing compounds. |
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