商陆抗病毒蛋白cDNA的克隆,测序及其植物表达载体的构建

商陆抗病毒重【'1（m）卜。。）（1。Ill'l－。l。。111。。。""。'，NP）是从美洲商陆叶片。I。分或Utoot件如VR，分子量约30hi）L'。PAP属典型的中链核栩然大活主l'I，对动物病毒和植物病；每均计）'们的抗病；沙作用'，'－。在国外，PAP达国已被川于如N...加'1物从因?..

Cloning and Sequencing of the cDNA Encoding for Pokeweed Anti-viral Protein (PAP) and Construction of Its Plant Expression Vector

The total RNA was extraeted from the leaves of pokeweed (phytolacca americana L.) plants whichhad been subcultured for eight weeks. The first stran of eDNA was synthesized from the total RNA templatewith oligo(dT) 15 primer using MMLV reverse transeriptase.A 0. 96 kb cDNA fragment was obtained after 30PCR amplification cycles with two speeifie primers. The cDNA fragment was sequenced from from directions after being cloned into pGEM-T Easy Veetor.The result showed that the cDNA clone had the entire coding region with the same sequence as the previously published pokeweed anti-viral protein (PAP)cDNA clone. Theplant expression vector with the PAP cDNA was eonstrueted,and the work of transfekrring the PAP cDNA intotobacco and rape obtain transgenie plants resistant to a broadspectrum of viruses in in progress.