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Study of the interaction of cadmium with membrane-bound succinate dehydrogenase |
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| Authors: | David Jay Rafael Zamorano Eduardo Muñoz Roberto Gleason José Luis Boldu |
| Institution: | (1) Departamento de Bioquimica, Instituto Nacional de Cardiología, Ignacio Chávez, Mexico;(2) Departamento de Física, Escuela Superior de Física y Matemáticas, IPN, Mexico;(3) Instituto de Física, Universidad Nacional Autónoma de México, Mexico;(4) Departamento de Bioquímica, Instituto Nacional de Cardiología, Ignacio Chávez, Juán Badiano No. 1, D.F. 14080 México, Mexico |
| Abstract: | Cadmium ions inhibit membrane-bound succinate dehydrogenase with a second-order rate constant of 10.42 mM–1s–1 at pH 7.35 and 25°C. Succinate and malonate protect the enzyme against cadmium ion inhibition. The protection pattern exerted by succinate and malonate suggests that the group modified by cadmium is located at the active site. The pH curve of inactivation by Cd2+ indicates the involvement of an amino acid residue with pKa of 7.23. |
| Keywords: | Mitochondria phosphorylating electron transport particles succinate dehydrogenase heart succinate dehydrogenase respiratory chain cadmium substrate-binding site |
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