LARGE SCALE PREPARATION OF A CRUDE MEMBRANE FRACTION FROM OX BRAIN |
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| Authors: | R. Rodnight M. Weller P. S. G. Goldfarb |
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| Affiliation: | Department of Biochemistry, Institute of Psychiatry (British Postgraduate Medical Federation, University of London) De Grespigny Park, London S.E.5 |
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| Abstract: | Abstract— Two procedures are described for preparing from ox brain grey matter a subcellular fraction enriched in neuronal membranes. In the first, an angle rotor was used to fractionate the crude mitochondrial and nuclear fraction and yielded 1·3–1·4 g of membrane protein from 200 g of tissue. The second procedure employed essentially the same principle except that a zonal rotor was used to fractionate in the final stage. The two methods gave comparable results for yields of protein, Na-K-Mg AT Pase and succinate dehydrogenase. |
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