Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules |
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Authors: | Rajan Binoy Fernandes Jorge M O Caipang Christopher M A Kiron Viswanath Rombout Jan H W M Brinchmann Monica F |
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Institution: | Aquatic Animal Health Research Group, Faculty of Biosciences and Aquaculture, University of Nordland, 8049 Bod?, Norway. |
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Abstract: | The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC-MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR. |
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