Denture disinfection by microwave energy: influence of Candida albicans biofilm

doi: 10.1111/j.1741‐2358.2010.00439.x Denture disinfection by microwave energy: influence of Candida albicans biofilm Objective: This study evaluated the influence of the area of Candida albicans biofilm on denture disinfection by microwave energy. Materials and methods: Candida albicans biofilm was allowed to form for 72 h on resin discs, and three small coverage or seven large coverage discs were placed onto the palatal surface of sterile maxillary dentures. Each denture was immersed in 200 ml distilled water and individually irradiated at a power of 450, 630 or 900 W for different time intervals (1, 2 or 3 min) (n = 6). The effectiveness of disinfection was evaluated by counting the residual cells. The data were analysed by anova and Tukey’s HSD test (α = 0.05). Pearson’s correlation test was performed to determine the correlation between effectiveness of sterilisation and temperature. Results: Dentures with a larger area of biofilm demanded a longer irradiation exposure to achieve disinfection (p < 0.001), irrespective of power setting, and in this time no yeast growth was detected. Dentures with small areas of biofilm were disinfected after 1 min at 900 W and 2 min at 450 or 630 W. A positive correlation was found between water temperature and effectiveness of disinfection (r = 0.6170; p < 0.001). Conclusion: The C. albicans biofilm area influenced disinfection by microwave energy; therefore dentures with larger biofilm areas required longer irradiation exposure to be disinfected.