Reversible immobilization of Candida rugosa lipase on fibrous polymer grafted and sulfonated p(HEMA/EGDMA) beads |
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Authors: | M Yakup Arica Hande Soydogan Gulay Bayramoglu |
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Institution: | (1) Biochemical Processing and Biomaterial Research Laboratory, Faculty of Arts and Sciences, Gazi University, Teknik Okullar, 06500 Ankara, Turkey |
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Abstract: | Poly(2-hydroxyethyl methacrylate/ethylenglycol dimethacrylate) beads were grafted with poly(glycidylmethacrylate) via surface
initiated atom transfer radical polymerization. Epoxy groups of the grafted polymer were modified in to sulfone groups. Sulfonated
beads were characterized by swelling studies, FT-IR, SEM and elemental analysis, and were used for reversible immobilization
of lipase. Under given experimental conditions, the beads had an adsorption capacity of 44.7 mg protein/g beads. The adsorbed
lipase on beads retained up to 67.4% of its initial activity. The immobilized lipase exhibited improved thermal and storage
stabilities over those of the free enzyme. The immobilized lipase could desorb 1.0 M NaCl solution at pH 8.0, and the sulfonated
beads can be repeatedly charged with fresh enzyme after inactivation upon use. |
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