Native granule associated short chain length polyhydroxyalkanoate synthase from a marine derived <Emphasis Type="Italic">Bacillus</Emphasis> sp. NQ-11/A2 |
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Authors: | Nimali N Prabhu Maria Celisa Santimano Suneela Mavinkurve Saroj N Bhosle Sandeep Garg |
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Institution: | (1) Department of Microbiology, Goa University, Taleigao Plateau, Goa, 403 206, India |
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Abstract: | A rapidly growing marine derived Bacillus sp. strain NQ-11/A2, identified as Bacillus megaterium, accumulated 61% polyhydroxyalkanoate by weight. Diverse carbon sources served as substrates for the accumulation of short
chain length polyhydroxyalkanoate. Three to nine granules either single or attached as buds could be isolated intact from
each cell. Maximum activity of polyhydroxyalkanoate synthase was associated with the granules. Granule-bound polyhydroxyalkanoate
synthase had a Km of 7.1 × 10−5 M for DL-β-hydroxybutyryl-CoA. Temperature and pH optima for maximum activity were 30°C and 7.0, respectively. Sodium ions
were required for granule-bound polyhydroxyalkanoate synthase activity and inhibited by potassium. Granule-bound polyhydroxyalkanoate
synthase was apparently covalently bound to the polyhydroxyalkanoate-core of the granules and affected by the chaotropic reagent
urea. Detergents inhibited the granule-bound polyhydroxyalkanoate synthase drastically whilst glycerol and bovine serum albumin
stabilized the synthase. |
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