Influence of Sambucus nigra bark lectin on cell DNA under different in vitro conditions |
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Authors: | Macewicz Larysa L Suchorada Olena M Lukash Lyubov L |
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Affiliation: | Department of Human Genetics, Institute of Molecular Biology and Genetics, NAS of Ukraine, Zabolotnoho street, 150, Kyiv 03143, Ukraine. |
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Abstract: | The biological activity of Sambucus nigra bark lectin on Chinese hamster cells in vitro was investigated by comet-assay and cytotoxicity testing. Mitogenic properties at the concentrations 0.063-0.25 microg/ml (but not higher) were found, and the induction of DNA breaks at concentrations 0.5 microg/ml and higher is demonstrated. S. nigra bark lectin at mitogenic concentrations decreased the level of nickel-induced DNA damage. The character and mechanism of this lectin protective activity was probably related to the induction of DNA reparation in the cells, decreasing nickel uptake in cells, and non-specific binding of nickel ions by protein molecules. |
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Keywords: | Lectin DNA breakage Nickel chloride Mutagenic activity Cytotoxicity Comet‐assay Mammalian cells in vitro |
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