Detection and verification of quantitative trait loci for resistance to Fusarium ear rot in maize |
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Authors: | Jiafa Chen Junqiang Ding Huimin Li Zhimin Li Xiaodong Sun Jingjing Li Ruixia Wang Xiaodong Dai Huafang Dong Weibin Song Wei Chen Zongliang Xia Jianyu Wu |
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Affiliation: | 1. College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China 2. College of Life Sciences, Henan Agricultural University, Wenhua Road, Jinshui, Zhengzhou, 450002, China
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Abstract: | Fusarium ear rot caused by Fusarium verticillioides is a prevalent disease in maize which can severely reduce grain yields and quality. Identification of stable quantitative trait loci (QTL) for resistance to Fusarium ear rot is a basic prerequisite for understanding the genetic mechanism of resistance and for the use of marker-assisted selection. In this study, two hundred and ten F 2:3 families were developed from a cross between resistant inbred line BT-1 and susceptible inbred line Xi502, and were genotyped with 178 simple sequence repeat markers. The resistance of each line was evaluated in two environments by artificial inoculation using the nail-punch method. The resistance QTL were detected using the composite interval mapping method. Three QTL were detected on chromosomes 4, 5 and 10. Of them, the QTL on chromosome 4 (bin 4.05/06) had the largest resistance to Fusarium ear rot, and could explain 17.95?% of the phenotypic variation. For further verification of the QTL effect, we developed near-isogenic lines (NILs) carrying the QTL region on chromosome 4 using parental line Xi502 as the recurrent parent. In the NIL background, this QTL can increase the resistance by 33.7?C35.2?% if the resistance region is homozygous, and by 17.8?C26.5?% if the resistance region contains the heterozygous allele. The stable and significant resistance effect of the QTL on chromosome 4 lays the foundation for further marker-assisted selection and map-based cloning in maize. |
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