QTL analysis for pseudostem pungency in bunching onion (Allium fistulosum) |
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Authors: | Hikaru Tsukazaki Shigenori Yaguchi Ken-ichiro Yamashita Masayoshi Shigyo Akio Kojima Tadayuki Wako |
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Institution: | 1. NARO Institute of Vegetable and Tea Science (NIVTS), National Agriculture and Food Research Organization, 360 Ano-Kusawa, Tsu, Mie, 514-2392, Japan 2. Research Fellow of Japan Society for the Promotion of Science, 8 Ichibancho, Chiyoda-ku, Tokyo, Tokyo, 102-8472, Japan 3. Faculty of Agriculture, Department of Biological and Environmental Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, Yamaguchi, 753-8515, Japan
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Abstract: | Low pungency is one of the most important agronomic traits in bunching onion (Allium fistulosum L.). Although the degree of pungency can be evaluated indirectly using a colorimetric test for pyruvic acid, DNA markers linked to low-pungency quantitative trait loci (QTLs) are still desired. In this study, we evaluated pungency in the bunching onion pseudostem through six trials conducted over 3?years using an F 2:3 population. QTL analysis based on the genetic linkage map revealed that the major pungency QTL was located within a 24.2-cM interval on Chr. 2a. The low-pungency parent-derived allele at AFAT04B03, a simple sequence repeat locus linked to the pungency QTL, was rare among commercial bunching onion cultivars. In addition, individuals homozygous for the low-pungency parent-derived allele at AFAT04B03 were significantly less pungent than those that were homozygous or heterozygous. Thus, these findings suggest that AFAT04B03 is an effective selection marker for low pungency in bunching onion breeding. |
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