Flow cytometry as a tool for analyzing changes in Plasmodium falciparum cell cycle following treatment with indol compounds |
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Authors: | Schuck Desirée Cigaran Ribeiro Ramira Yuri Nery Arthur A Ulrich Henning Garcia Célia R S |
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Institution: | Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de S?o Paulo, S?o Paulo, Brazil. |
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Abstract: | Melatonin and its derivatives modulate the Plasmodium falciparum and Plasmodium chabaudi cell cycle. Flow cytometry was employed together with the nucleic acid dye YOYO-1 allowing precise discrimination between mono- and multinucleated forms of P. falciparum-infected red blood cell. The use of YOYO-1 permitted excellent discrimination between uninfected and infected red blood cells as well as between early and late parasite stages. Fluorescence intensities of schizont-stage parasites were about 10-fold greater than those of ring-trophozoite form parasites. Melatonin and related indolic compounds including serotonin, N-acetyl-serotonin and tryptamine induced an increase in the percentage of multinucleated forms compared to non-treated control cultures. YOYO-1 staining of infected erythrocyte and subsequent flow cytometry analysis provides a powerful tool in malaria research for screening of bioactive compounds. |
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