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In situ extraction of RNA from marine-derived fungi associated with the marine sponge, Haliclona simulans |
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| Authors: | Paul W. Baker Alan D. W. Dobson Julian Marchesi |
| Affiliation: | 1. School of Environment, Natural Resources and Geography, Bangor University, Bangor, Gwynedd, LL57 2UW, UK 2. Environmental Research Institute, University College Cork, Lee Road, Cork, Ireland 3. School of Biosciences, Museum Avenue, Cardiff University, Cardiff, CF10 3AT, UK |
| Abstract: | Several different methods were compared to recover a significant quantity of high quality RNA from H. simulans containing RNA of marine-derived fungi. Further processing of the RNA showed that amplification products could be obtained using fungal specific primers, RNA enriched in mRNA could be recovered and an oligonucleotide-linker could be attached to cDNA. The potential applications of these methods are discussed for future studies in determining whether marine fungi could be symbiotically associated with marine sponges. |
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