高脂饲料诱导的肥胖大鼠网膜素水平的实验研究

人群调查发现肥胖人群网膜素水平较正常人群低,而正常及肥胖大鼠血清网膜素水平及其基因表达情况尚不清楚.将SD大鼠随机分为正常组(n=10)和高脂组(n=30),分别喂养普通饲料和高脂饲料.6 w后从高脂组选取体重增长最快的20只,再从中随机抽取10只继续喂养高脂饲料,12 w后两组各剩9只,采用全自动生化仪ADVIA2400测定血糖及血脂、ELISA检测血清胰岛素及网膜素水平、RT-PCR检测网膜脂肪组织网膜素mRNA表达水平.结果显示高脂组大鼠体重、体重增加值、肥胖指数、低密度脂蛋白、胰岛素、血清网膜素水平及网膜脂肪组织网膜素mRNA表达水平均高于正常组(P<0.05).首次发现肥胖大鼠血清网膜素水平及网膜脂肪组织中网膜素mRNA表达水平较正常大鼠显著增高,与人群调查结果不一致.

Increased Serum Omentin and Its mRNA Expression in High-fat Diet Induced Obese Rats

Obese populations have significantly lower plasma omentin levels than lean ones,but the serum omentin level and its mRNA expression in omental adipose tissue in normal and high fat diet-induced obese rats is not clear.Male adult Sprague-Dawley rats were randomly divided into 2 groups: 10 rats in control group fed with normal diet,30 rats in experimental group with high fat diet(HFD).After 6 weeks,10 rats were randomly chosen from the top 20 rats with the highest weight gain.The experimental rats(n=10) were fed with HFD for another 6 weeks,after 12 weeks,only 9 rats were left in each group.At the end,blood samples were taken for the measurement of serum lipids,glucose,insulin and omentin.Adipose tissue sam-ples were taken for determination of the mRNA expression of omentin by RT-PCR.The results showed that after 12 weeks of high fat diet feeding,body weight,weight gain,the obesity index,LDL,insulin,serum o-mentin level and its mRNA expression in omental adipose tissue were significantly higher in HFD group rats than those in control group(P<0.05 for all comparisons).It is the first time to document that serum omentin and its mRNA expression in omental adipose tissue are increased in high fat diet-induced obese rats.It was inconsistent with the results found in population-based studies.