Skim milk enhances the preservation of thawed -80 degrees C bacterial stocks |
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Authors: | Cody William L Wilson James W Hendrixson David R McIver Kevin S Hagman Kayla E Ott C M Nickerson Cheryl A Schurr Michael J |
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Institution: | Department of Microbiology, University of Colorado Denver, School of Medicine, Aurora, CO 80045, USA. |
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Abstract: | The results from bacterial strain recovery efforts following hurricanes Katrina and Rita are reported. Over 90% of strains frozen in 10% skim milk were recovered whereas various recovery rates were observed for glycerol-stored stocks (56% and 94% of Escherichia coli, depending upon the laboratory). These observations led to a viability comparison of Streptococcus pyogenes, Campylobacter jejuni, Borrelia burgdorferi, Salmonella enterica subsp. Typhimurium, Pseudomonas aeruginosa and E. coli strains stored in glycerol or skim milk. In all bacteria examined, 10% skim milk resulted in significantly longer viability after thawing than 15% glycerol solutions currently used in most laboratories. |
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Keywords: | Cryopreservation Skim milk Bacterial strain Glycerol |
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