人胸腺上皮细胞培养及其分泌产物

本文通过降低培养基中血清含量,向RPMI 1640培养基中补加三碘甲腺原氨酸而获得一种人胸腺网状上皮细胞占优势生长的培养物。在此培养基中细胞经传代培养长达90天,仍维持正常形态特征。胸腺组织在培养14天后,新生细胞的突起形成网状结构,细胞化学检查和电镜观察表明具有丰富的分泌颗粒,囊泡及张力原纤维束和桥粒等上皮细胞特征。收集合并细胞培养液,经部分纯化后检查其生物活性,表现出具有促进玫瑰花结形成和降低胸腺细胞TdT活性的作用,说明培养细胞的分泌产物具有胸腺激素活性。根据形态学,细胞化学和生物活性检测结果,我们倾向于认为该培养物主要为网状上皮细胞。

CULTURE OF HUMAN THYMIC EPITHELIAL CELLS AND THEIR SECRETORY PRODUCTS

A human thymic culture dominated by reticulo-epithelial cells was obtained by re-ducing the calf serum concentration and ad-ding triiodothyronine(T_3) to the RPMI 1640 medium.The thymic epithelial subculture cells keep their normal morphological characters within a duration of 90 days in this modified medium.The proliferating cells formed the re-ticular structure which appeared at the 14 th day.The cytochemical and electron microsco-pic observation indicated that most of the cul-tured cells contain a great deal of secretory granuls,vesicles,tonofibrils and desmosomes showing the characters of epithelial cells. All media collection at different time with 3-4 days interval were mixed.The thy-mosin was partially purified from the secre-tion with a bioassay.The results showed tha the cell products have activities to increase the number of rosette forming cells and decrease the TDT level of thymocytes,these facts in-dicated that the cell products have the activi-ty of thymic hormone. According to the facts of morphology,cytochemistry and bioassays,we incline to consider that it is a thymic reticulo-epithelial cells dominated culture.